DOT Urine Specimen Collector Training Online
Complete your DOT urine specimen collector qualification training and 5 required mock collections in one $299 course. Supplies are included if needed.
No separate mock collection package required.
Includes training, 5 mock collections, and supplies if needed.
Current with 2026 DOT guidance
Reviewed and updated for DOT’s June 2026 Urine Specimen Collection Procedures Guidelines and the revised 2026 Federal CCF.
- Trusted by 28,000 medical professionals
- 5 Mock collections included
- Supplies included if needed
- Certificate after successful completion
What’s Included in the $299 Price?
Qualification Training
8 training videos (83 mins total) including seven DOT qualification modules and a non-DOT overview
5 Required Mock Collections Included
Complete your DOT proficiency demonstration by performing 5 mock collections via video conference
Collection Kits for Mock Collections
If needed, we will mail you the supplies needed for your mock collections
Certificate of Completion
Available upon review of your completed CCFs from the mock collections
Avoid Surprise Add-On Fees
Some DOT collector courses advertise a low upfront price, but required mock collections, supplies, or proficiency demonstration fees may cost extra. TeamCME includes the qualification training, 5 mock collections, and supplies if needed in one transparent price.
| Feature |  | Some Lower-Priced Courses |
|---|---|---|
| Online qualification training | Included | Usually included |
| 5 mock collections | Included | May cost extra |
| Supplies if needed | Included | May cost extra |
| Downloadable manual | Included | Varies |
| Certificate after successful completion | Included | Varies |
| Group registration help | Available | Varies |
Training 3 or More Collectors? Ask About Group Pricing
Need to train several staff members at the same clinic, company, or organization? TeamCME offers group registration for clinics and teams registering 3 or more students for DOT urine specimen collector training.
Who This Course is For
This course is designed for individuals and organizations that need DOT urine specimen collector training, including:
- New collectors needing DOT drug test training & mock collections
- Existing collectors needing 5-year refresher training
- Clinics adding DOT drug testing services
- Occupational health clinics training staff members
- Mobile drug testing businesses
- Employers who want in-house staff trained to perform collections
How it Works
Complete your DOT urine specimen collector training in 5 simple steps.
1. Register Online
Enroll in the course and get access to the online training materials.
2. Complete the Training Videos
Watch the self-paced qualification training videos on your schedule.
3. Pass the Quiz
Complete the 32-question quiz. You may retake it as needed to pass with 80% or higher.
4. Schedule Your Mock Collections
After passing the quiz, schedule your live video proficiency demonstration.
5. Receive Your Certificate
Your certificate is issued after successful completion of the training, quiz, 5 mock collections, and document review.
Ready to get started?
What You’ll Learn
Learn the essential procedures and compliance requirements for performing DOT urine specimen collections, from preparing the collection site through specimen shipment, including:
- DOT regulations, collector responsibilities, qualification requirements, and professional conduct
- Collection-site preparation, security procedures, and proper completion of the Federal Custody and Control Form (CCF)
- Step-by-step urine collection procedures, specimen integrity checks, bottle sealing, and laboratory shipment
- Managing insufficient specimens (shy bladder), unusual medical situations, and suspected specimen tampering
- Requirements for monitored and directly observed collections, including privacy, documentation, and refusal procedures
- Preventing and correcting collection errors, identifying fatal flaws, and understanding how DOT and non-DOT testing differ
Curriculum
Our comprehensive training program includes seven DOT urine specimen collector qualification modules and an eighth module covering non-DOT drug testing. Together, the eight videos provide approximately 83 minutes of instruction, including practical examples and knowledge checks.
After completing the required training, students take a 48-question assessment and participate in a live proficiency demonstration consisting of five consecutive error-free mock collections.
Module 1: Foundations, Roles, and Collector Qualification - 13 min 31 sec
- Overview of 49 CFR Part 40, current DOT urine collection guidelines, and applicable DOT agency regulations
- The six reasons for DOT-required drug testing and the transportation industries subject to testing
- Responsibilities of the collector, employer, Designated Employer Representative (DER), Medical Review Officer (MRO), laboratory, and consortium/third-party administrator (C/TPA)
- Collector qualification requirements under §40.33, including initial training, five required mock collections, five-year refresher training, and error-correction training
- Collector restrictions, conflicts of interest, and professional responsibilities under §40.31
- Maintaining collector qualification documentation, privacy, and specimen integrity
Module 2: Collection Sites, Security, Supplies, and the 2026 CCF - 9 min 39 sec
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Requirements for acceptable collection sites, including medical facilities, mobile locations, and employer worksites
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Preparing single-stall and multi-stall restrooms to protect privacy and prevent specimen tampering
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Securing water sources, removing potential adulterants, and controlling access to the collection area
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Required collection supplies, specimen containers, tamper-evident seals, and shipping materials
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Understanding the updated Federal Drug Testing Custody and Control Form (CCF)
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Verifying employer, laboratory, MRO, employee, and collection-site information before beginning a collection
Module 3: Routine Collection I - Arrival Through Specimen Receipt - 11 min 13 sec
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Beginning the collection promptly and verifying the employee’s identity
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Confirming the testing instructions and appropriate information on the CCF
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Preparing the employee for collection, including handling personal belongings, emptying pockets, and addressing suspicious items
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Providing clear instructions for specimen collection while protecting employee privacy
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Receiving the specimen and performing the three immediate checks: specimen volume, temperature, and appearance
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Recognizing insufficient specimens, out-of-range temperatures, and evidence of possible tampering
Module 4: Routine Collection II - Split, Seal, Complete, and Ship - 7 min 4 sec
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Dividing the urine specimen into the primary Bottle A (at least 30 mL) and split Bottle B (at least 15 mL)
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Correctly applying tamper-evident bottle seals, recording dates, and obtaining employee initials
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Handling damaged, reversed, or improperly applied specimen seals
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Completing the employee certification (Step 5) and collector certification (Step 4) on the Federal CCF
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Proper distribution of the five CCF copies to the laboratory, MRO, collector, employer, and employee
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Packaging, securing, storing, and shipping specimens while maintaining chain of custody, including applicable electronic CCF procedures
Module 5: Insufficient Specimen and Unusual Collection Situations - 7 min 23 sec
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Recognizing an insufficient specimen and properly initiating the three-hour shy-bladder procedure
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Managing the permitted fluid intake of up to 40 ounces over three hours and documenting collection attempts
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Maintaining employee supervision and preventing the combining of urine from separate collection attempts
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Responding when an employee cannot provide a sufficient specimen within the allowed time
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Distinguishing an inability to provide a specimen from behavior that may constitute a refusal to test
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Handling unusual medical collection situations, including self-catheterization, external urine collection bags, and required privacy accommodations
Module 6: Monitored, Directly Observed, Temperature, and Tampering - 8 min 55 sec
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Understanding the differences between monitored collections and directly observed collections
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Recognizing the circumstances requiring direct observation, including return-to-duty and follow-up testing, certain MRO-directed collections, and suspected tampering
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Observer and monitor qualification requirements, including applicable same-sex requirements and exceptions
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Performing directly observed collections correctly while protecting employee privacy and dignity
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Responding to out-of-range specimen temperatures, unusual specimen characteristics, and suspected substitution or adulteration
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Completing and documenting a second directly observed collection when required, including properly linking separate CCFs
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Addressing employee refusals, observation problems, and communication with the DER
Module 7: Corrections, Fatal and Correctable Flaws, Records, and Course Completion - 9 min 2 sec
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Identifying the seven fatal flaws that require cancellation of a DOT urine drug test
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Distinguishing fatal flaws, correctable flaws, and other collection or documentation errors
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Correcting CCF errors appropriately, including preparing signed correction statements and memoranda when required
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Recognizing when a collector must complete error-correction training following a cancelled test
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Maintaining required collection records, safeguarding employee information, and retaining collector qualification documentation
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Reviewing the complete collection procedure and understanding the final assessment and five-mock-collection proficiency requirements
Module 8: Non-DOT Drug Testing Overview - 16 min 19 sec
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Understanding the differences between DOT-required testing and employer-policy non-DOT drug testing
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Keeping DOT and non-DOT specimens, collection forms, testing procedures, and results separate
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Using rapid, point-of-collection urine tests, including when they may be appropriate and how preliminary results are interpreted
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Understanding laboratory screening, confirmation testing, specimen-validity testing, and the meaning of presumptive non-negative and laboratory-confirmed positive results
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Understanding the role of the MRO in reviewing laboratory results, contacting donors, and evaluating legitimate prescription explanations
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Communicating testing information accurately while distinguishing the responsibilities of collectors, laboratories, MROs, and employers.
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Reviewing non-DOT laboratory-first and rapid-screen testing workflows, along with instructions for obtaining the TeamCME certificate after completing the required mock collections
Specimen Collector Assessment Quiz - 48 Questions
Students must complete the 48-question assessment with a passing score of 80% or higher. The quiz may be retaken as many times as needed to achieve a passing score.
Five Required Mock Collections - Approximately 90 Minutes
Students complete a live proficiency demonstration by video conference, performing five consecutive error-free mock collections under the observation of a qualified evaluator.
The demonstration includes:
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Two uneventful routine urine collections
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One insufficient specimen (shy bladder) scenario
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One out-of-range specimen temperature scenario
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One combined refusal to sign the CCF and initial the specimen bottle seals
Students can schedule their mock collections through the online training portal after passing the assessment. Required collection supplies are included if needed.
After successfully completing the proficiency demonstration, students submit their completed CCFs for TeamCME review. Once reviewed and approved, the certificate becomes available through My Certificates in the training portal.
Built by DOT Compliance Training Experts
TeamCME has trained medical professionals nationwide in DOT compliance, including NRCME medical examiner training and drug and alcohol testing education. Our training programs are developed with a practical understanding of DOT procedures, documentation, and compliance expectations.
TeamCME founder Dr. Michael Megehee has been training medical professionals to perform DOT compliance services since 1996. In fact, he was selected by the Federal Motor Carrier Safety Administration (FMCSA), an agency of the DOT, as a subject matter experts who helped design training curriculum.
Dr. Jeff Carlson, TeamCME’s Chief Medical Officer, co-developed this course and leads the development of TeamCME’s training programs. He has published on DOT medical compliance and has trained medical professionals nationwide through live seminars.
FAQs
Is this course updated for the June 2026 DOT guidelines and revised Federal CCF?
Yes. TeamCME’s course has been reviewed and updated to reflect DOT’s June 2026 Urine Specimen Collection Procedures Guidelines, related Part 40 changes, and the revised 2026 Federal CCF. It includes current procedures for CCF completion and directly observed urine collections.
What is included in the $299 DOT specimen collector training?
The $299 course includes DOT urine specimen collector qualification training, a downloadable training manual, a quiz, 5 mock collections completed by live video conference, and a certificate of completion after your mock collection documents are reviewed and approved. Collection supplies are also included if needed.
Are mock collections included?
Yes. The course includes the required 5 mock collections, also called the proficiency demonstration. These are completed by live video conference after you finish the training videos and pass the quiz.
Are collection supplies included?
Yes. If you need supplies for your mock collections, TeamCME will mail collection kits to you. This helps you complete the mock collection portion of the training without having to find supplies on your own.
How are the 5 mock collections completed?
After completing the training videos and passing the quiz, you can schedule your mock collections through our online scheduling system. The mock collections are completed by live video conference. During the session, you will demonstrate the collection process and complete the required paperwork for review
How long does the course take?
The eight qualification videos total approximately 83 minutes. The course also includes a 48-question quiz and a live five-mock-collection proficiency demonstration, typically lasting about 90 minutes.
Can I complete the training at my own pace?
Yes. The course and all its content is available at any time. When you pause your training, the course will keep track of where you left off. Even after completion, you can go back and review the material.The course is accessible for one year from your registration date.
When do I receive my certificate?
Your certificate is issued after you successfully complete the training, pass the quiz, complete the 5 mock collections, and have your mock collection documents reviewed and approved. It can be found in your training portal upon completion.
Can this course be used for 5-year recertification?
Yes. This course may be used for collectors who need 5-year requalification training. It includes both the training content and the proficiency demonstration with 5 mock collections.
Do you offer group pricing for clinics or teams?
Yes. If you need to train three or more collectors, contact us about group registration options. TeamCME can help clinics, companies, and organizations enroll multiple students.
Is this course for DOT urine specimen collectors?
Yes. This course is designed for DOT urine specimen collectors. It covers DOT urine collection procedures, chain of custody form completion, specimen integrity, common errors and fatal flaws, shy bladder protocol, observed and monitored collections, and other topics collectors need to understand for DOT-compliant collections.
Is this the same as DOT drug testing certification?
Many people search for DOT drug testing certification when they are looking for DOT urine specimen collector training. This course is designed to provide DOT urine specimen collector qualification training, including the required 5 mock collections, also called the proficiency demonstration.
